Direct Fluorescent Labeling of Live Bacterial Cells
Bacterial resistance is a rapidly emerging public health threat that underscores the need for development of novel antibiotic agents with new modes of action. The bacterial cell wall has been an attractive target for the development of antibiotics, but there are limited methods that allow direct visualization of active sites of cell growth and no methods to assess antimicrobial activity in situ. Recently, Professor Michael VanNieuwenhze and Professor Yves Brun (Biology) have developed a one-step method to label zones of growth in live bacterial cells. The method relies upon the ability of the bacterial cell to incorporate fluorescently-modified D-amino acids into its cell wall. This method has been applied to a broad array of evolutionarily diverse bacteria and it provides a powerful new tool for development of a better understanding of the basic mechanisms of bacterial growth and how it may be modified in response to environmental factors.
Distinguished Professor
Robert & Marjorie Mann Chair
Distinguished Professor
Linda & Jack Gill Chair in Biomolecular Science
James F. Jackson Professor of Chemistry
Class of 1948 Herman B Wells Endowed Professor
Adjunct Professor, Physics
Associate Dean of Natural and Mathematical Sciences and Research
Associate Professor (O'Neill School of Public and Environmental Affairs),
Adjunct Professor (Chemistry)
Rudy Professor (O'Neill School of Public and Environmental Affairs)
Adjunct Professor (Chemistry)
Professor & Associate Vice President for Engagement
Professor, Department of Molecular and Cellular Biochemistry
Adjunct Professor