- METACyt Biomolecular NMR Laboratory
- Macromolecular Crystallography and Crystallization Automation Facilities
- Nanoscale Characterization Facility
- Mass Spectrometry
- Molecular Structure Center
- Nuclear Magnetic Resonance Facility
- Electron Paramagnetic Resonance Room
- Physical Biochemistry Instrumentation Facility
- NanoTemper Monotlith NT.115 Blue/Red Microscale Thermophoresis
- Perkin-Elmer LS50B Luminescence Spectrometer
- Beckman Coulter Optima XL-I Analytical Ultracentrifuge
- Microcal VP-ITC Isothermal Titration Calorimeter
- Amersham Biosciences Typhoon 9210 Variable Mode Imager
- Jasco J-715 Circular Dichroism Spectropolarimeter
- Varian Cary 100 Bio UV / Visible Spectrometer
- Malvern Instruments Zetasizer Nano-S Dynamic Light Scatterer
- BIACORE 3000
- Packard 1600TR Liquid Scintillation Counter
- KinTek SF-300X Stopped Flow Spectrometer
- Wyatt Technology DAWN HELEOS II (MALS)/OptiLab rEX Multi-angle Light Scattering Instrument and Refractive Index Detector
- Applied Biosystems StepOne Plus RT-PCR Real-time Polymerase Chain Reaction Instrument
- Beckman XPN Preparative Ultracentrifuge
Nanoscale Characterization Facility
The Nanoscale Characterization Facility (NCF) is housed on the ground floor in Simon Hall, our multidisciplinary sciences building, and has 2000 ft2 of laboratory space and 1300 ft2 of cleanroom space. The NCF provides faculty, staff, postdoctoral fellows, and graduate and undergraduate students with state-of-the-art instrumentation for generating and characterizing materials having features with nanometer dimensions. We expect the NCF to grow over the next three to five years as a university resource, and the instrumentation within the facility will be available to all research groups at IU.
The MSF is located in A411 and is staffed from 9:30 to 5:30 daily and facility houses the following equipment: One Thermo MAT-95 XP magnetic sector mass spectrometer (EI and CI sources available, staff use only), two Waters LCT Classic electrospray/time-of-flight LC-MS systems (available for walk-up use), one Bruker Biflex III MALDI-TOF instrument (available for walk-up use), one Agilent 6890/5973 GC-MS (available for walk-up use), and one soon to be installed Agilent 1200/6310 ESI-quadrupole LC-MS system (available for walk-up use). See Dr. Jonathan Karty (facility manager) or Angela Hansen (Senior Mass Spectroscopist) for more information or to schedule training. Rates for IU faculty research groups can be found on the Instrumentation page of the MSF website.
- Walk-up electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for both small molecules and biological samples
- Training for the IU community in the theory and practice of modern mass spectrometry
- High resolution, high accuracy (±5 parts per million, ppm) mass spectrometry of small molecules by electron impact, chemical ionization, and electrospray ionization
- High mass resolution, high mass accuracy gas chromatographic mass spectrometry
- Low mass resolution gas chromatographic mass spectrometry with both electron impact and chemical ionization sources
- Capillary-scale (minimum column i.d. = 0.3 mm) high performance liquid chromatographic mass spectrometry (HPLC-MS) with simultaneous detection of up to two UV-VIS wavelengths
- Analysis of air and water sensitive compounds by electron impact, chemical ionization, fast atom bombardment, electrospray ionization, and matrix assisted laser desorption/ionization.
Molecular Structure Center
The Indiana University Molecular Structure Center laboratory has a full complement of single crystal and powder diffraction equipment used to characterize crystalline materials using the techniques of X-ray crystallography. Researchers in the laboratory can determine the three-dimensional structure of nearly any material that can be crystallized.
The results of a crystallographic study is a set of atomic coordinates which locate the atoms of a molecule in the "unit cell" of the crystal. This information can then be used to generate images of the molecule and to determine distances and angles in the molecule. In addition, the data allows one to examine the packing of the molecules in the crystal - information which can often lead to understanding the properties of the material.
IUMSC Server allows rapid access to the data that are generated in the IUMSC. Nearly all of the materials studied have been synthesized or isolated by researchers from other laboratories, usually within the Indiana University system, but often from laboratories throughout the world. This server supports these researchers to access to the data as soon as the study is complete.
Nuclear Magnetic Resonance Facility
The NMR Facility provides 7 Varian/Agilent high resolution NMR spectrometers in from 200 MHz to 800 MHz to perform all modern solution-state experiments. State-of-the-art 600 MHz and 800 MHz instruments with HCN cold probes dedicated to BioNMR are housed in the METACyt Biomolecular NMR Laboratory in Simon Hall. 500 MHz, 300 MHz and 2 400 MHz spectrometers reside in Chemistry for walkup use. A variety of probes are available for these instruments including HCN, HCX and HCNP and broadband tunable probes as well as the default (1H, 13C) and (1H, 19F, 13C, 31P) probes. All of these instruments have variable temperature systems. The 500 MHz instrument has a 3 axis gradient driver and probe for microimaging. A 200 MHz instrument is also available primarily for undergraduate instruction.
Workstations for offline use are provided in the Chemistry Facility. In addition the facility maintains site licenses for Mestrenova offline processing software for PC and Mac and ACD chemical shift prediction.
The NMR facility also features numerous training resources, a wet lab for sample preparation, a comprehensive web reservation system, easy remote access to data and data archiving.
Electron Paramagnetic Resonance Room
The Electron Paramagnetic Resonance Room provides support to research projects of the chemistry department as well as other departments in chemistry, physics, material science, and biology. Electron Paramagnetic Resonance is a spectroscopic technique for studying molecules that contain unpaired electrons, typically transition-metal ions and organic radicals, yielding structural and dynamic information. The EPR room is equipped with a Bruker EMX X-band EPR spectrometer with liquid-helium low temperature (down to 4K) accessories. Frank Gao is currently in charge of the spectrometer, with Professor Kenneth Caulton as the faculty adviser.
Physical Biochemistry Instrumentation Facility
Over the past several years there has been a rapid increase on the IUB campus of research into the structures, stabilities and interactions of biomolecules. The Physical Biochemistry Instrumentation Facility was established to facilitate and encourage these research endeavors and to provide a centralized resource for training and education in modern physical biochemistry. Located in the new, state-of-the-art Simon Hall, it houses state-of-the-art biophysical instrumentation that is available for use by faculty and students.