This is a high mass accuracy/high sensitivity instrument which is capable of obtaining MS spectra at 500,000 resolution. It is capable of fragmenting molecules using collision-induced dissociation (CID), electron transfer dissociation (ETD), and higher energy collisional dissociation (HCD). This makes it an ideal instrument for proteomics and protein/peptide characterization, particularly with respect to mapping post-translational modifications and sites of protein-protein crosslinking. Its high speed and sensitivity enable the identification of 1000s of proteins in a single LC-MS analysis. In addition, it can be used for the quantitative analysis of samples labeled with stable isotope techniques such as iTRAQ or TMT-labeling.
This is a high mass accuracy mass spectrometer equipped with an ion mobility cell. In addition to standard collision induced dissociation (CID), this instrument can also conduct fragmentation via electron transfer dissociation (ETD). Principle uses include metabolite profiling and identification, analysis of intact proteins, and identification of peptides and post-translationally modified peptides.
This mass spectrometer is the newest generation ion trap. While relatively low resolution, it has extremely fast scan speeds and low sensitivity. These characteristics make it ideal for characterization of protein digests ranging in complexity from in-gel digestions to whole cell lysates.
This is a hybrid triple quadrupole/linear ion trap mass spectrometer. It is used primarily to quantify previously characterized small molecules and peptides in a highly reproducible fashion.
AKTA Pure High Performance Liquid Chromatography (HPLC)
This is an HPLC designed for chromatography of biological samples. It can operate at flow rates between 10 μl and 10 ml/min. It is used for the fractionation of peptide mixtures using reverse phase chromatography or strong cation exchange chromatography. In addition, proteins can be purified or fractionated using size exclusion chromatography or cation exchange. It is a key component in our enrichment of phosphorylated or glycosylated peptides.